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mitochondria isolation buffer c  (Thermo Fisher)


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    Thermo Fisher mitochondria isolation buffer c
    Mitochondria Isolation Buffer C, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mitochondria+isolation+buffer/Sucrose/bio_rxiv__64898__2026__01__30__702752-233-15-44
    Average 98 stars, based on 1 article reviews
    mitochondria isolation buffer c - by Bioz Stars, 2026-09
    98/100 stars

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    Related Articles

    Isolation:

    Article Title: Identification of elongation factor‐2 as a novel regulator of mitochondrial fission
    Article Snippet: .. Cells were suspended in mitochondria isolation buffer (250 mM sucrose, 0.1 mM EDTA, and 2 mMHEPES, pH 7.4) containing protease inhibitors and phosphatase inhibitors (Thermo Scientific) and homogenized using 21G needle. ..

    Article Title: TMEM65 functions as the mitochondrial Na + /Ca 2+ exchanger.
    Article Snippet: Mitochondria export Ca via Na/Ca exchange machinery (mito-NCX) to regulate intracellular Ca signalling and mitochondrial Ca homeostasis.. TMEM65 has recently been implicated as essential for mito-NCX, but its mechanisms and roles remain unclear.. Here we show that TMEM65 depletion severely impairs mito-NCX.

    Article Title: Deletion of SUMO1 attenuates behavioral and anatomical deficits by regulating autophagic activities in Huntington disease
    Article Snippet: .. For subcellular fractionations, mice were euthanized and both striata were dissected immediately and homogenized using a glass Dounce homogenizer (5 loose and 5 tight strokes) in buffer A of mitochondria isolation buffer (ThermoFisher Scientific, no. 89874) and kept on ice for 2 minutes. .. For subcellular fractionations, mice were euthanized and both striata were dissected immediately and homogenized using a glass Dounce homogenizer (5 loose and 5 tight strokes) in buffer A of mitochondria isolation buffer (ThermoFisher Scientific, no. 89874) and kept on ice for 2 minutes.

    Article Title: Interaction of lncRNA LENT with DHX36 regulates translation and suppresses autophagy in melanoma
    Article Snippet: .. Then, purified mitochondria were digested on ice for 15 min with increasing concentrations of Digitonin (Invitrogen) in mitochondria isolation buffer (210 mM Mannitol, 70 mM Sucrose, 1 mM EDTA, 10 mM HEPES and protease inhibitors cocktail). .. Then, purified mitochondria were digested on ice for 15 min with increasing concentrations of Digitonin (Invitrogen) in mitochondria isolation buffer (210 mM Mannitol, 70 mM Sucrose, 1 mM EDTA, 10 mM HEPES and protease inhibitors cocktail).


    Article Title: Sex Differences in Response to Diet Enriched With Glutathione Precursors in the Aging Heart
    Article Snippet: .. The 2 pellets were resuspended in a mitochondria isolation buffer (70 mM sucrose, 210 mM mannitol, 0.5 mM HEPES, 0.1 mM EGTA, without BSA) containing a cocktail of 1X protease inhibitors and 1X phosphatase inhibitors (Pierce Protease and Phosphatase Inhibitor Mini Tablets, EDTA-free—Thermo Fisher Scientific #A32961). ..

    Article Title: Identification of Elongation Factor-2 as a Novel Regulator of Mitochondrial Fission
    Article Snippet: .. Cells were suspended in mitochondria isolation buffer (250 mM sucrose, 0.1 mM EDTA, and 2 mM HEPES, pH 7.4) containing protease inhibitors and phosphatase inhibitors (Thermo Scientific) and homogenized using 21G needle. ..

    Article Title: Sex Differences in Response to Diet Enriched with Glutathione Precursors in the Aging Heart.
    Article Snippet: © The Author(s) 2024.. Published by Oxford University Press on behalf of The Gerontological Society of America.. This is an Open Access article distributed under the terms of the Creative Commons AttributionNonCommercial License (https://creativecommons.org/licenses/by-nc/4.0/), which permits noncommercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited.

    Protease Inhibitor:

    Article Title: TMEM65 functions as the mitochondrial Na + /Ca 2+ exchanger.
    Article Snippet: Mitochondria export Ca via Na/Ca exchange machinery (mito-NCX) to regulate intracellular Ca signalling and mitochondrial Ca homeostasis.. TMEM65 has recently been implicated as essential for mito-NCX, but its mechanisms and roles remain unclear.. Here we show that TMEM65 depletion severely impairs mito-NCX.


    Purification:

    Article Title: Interaction of lncRNA LENT with DHX36 regulates translation and suppresses autophagy in melanoma
    Article Snippet: .. Then, purified mitochondria were digested on ice for 15 min with increasing concentrations of Digitonin (Invitrogen) in mitochondria isolation buffer (210 mM Mannitol, 70 mM Sucrose, 1 mM EDTA, 10 mM HEPES and protease inhibitors cocktail). .. Then, purified mitochondria were digested on ice for 15 min with increasing concentrations of Digitonin (Invitrogen) in mitochondria isolation buffer (210 mM Mannitol, 70 mM Sucrose, 1 mM EDTA, 10 mM HEPES and protease inhibitors cocktail).



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    mitochondria isolation buffer c  (Thermo Fisher)
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    mitochondria isolation buffer  (Thermo Fisher)
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    Thermo Fisher mitochondria isolation buffer
    A Immunofluorescence by confocal microscopy showing DHX36 localization in different cell lines. HSP60 is used as a <t>mitochondria</t> marker and DAPI to stain the nucleus. Scale bars = 10 µM. B DHX36 signal was quantified in the whole cell and divided by the signal in mitochondria or in the nucleus. Each measured cell is represented by one point, and groups are compared by one-way ANOVA (Dunnett test). * P < 0.033; ** P < 0.0021; *** P < 0.0002; **** P < 0.0001. C Immunoblot of cytosolic or mitochondrial fractions of different cell lines. D Purified mitochondria were digested with increasing concentrations of digitonin, and retained proteins were analyzed by western blot. E Immunogold staining coupled with electron microscopy. Representative stained particles for DHX36 or COX IV in the cytoplasm (C) or mitochondria (M) are indicated with arrows. Scale bars are indicated on images. F LENT levels quantified by RT-qPCR in cytosolic or mitochondrial fractions of different cell lines.
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    A Immunofluorescence by confocal microscopy showing DHX36 localization in different cell lines. HSP60 is used as a <t>mitochondria</t> marker and DAPI to stain the nucleus. Scale bars = 10 µM. B DHX36 signal was quantified in the whole cell and divided by the signal in mitochondria or in the nucleus. Each measured cell is represented by one point, and groups are compared by one-way ANOVA (Dunnett test). * P < 0.033; ** P < 0.0021; *** P < 0.0002; **** P < 0.0001. C Immunoblot of cytosolic or mitochondrial fractions of different cell lines. D Purified mitochondria were digested with increasing concentrations of digitonin, and retained proteins were analyzed by western blot. E Immunogold staining coupled with electron microscopy. Representative stained particles for DHX36 or COX IV in the cytoplasm (C) or mitochondria (M) are indicated with arrows. Scale bars are indicated on images. F LENT levels quantified by RT-qPCR in cytosolic or mitochondrial fractions of different cell lines.
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    Image Search Results


    A Immunofluorescence by confocal microscopy showing DHX36 localization in different cell lines. HSP60 is used as a mitochondria marker and DAPI to stain the nucleus. Scale bars = 10 µM. B DHX36 signal was quantified in the whole cell and divided by the signal in mitochondria or in the nucleus. Each measured cell is represented by one point, and groups are compared by one-way ANOVA (Dunnett test). * P < 0.033; ** P < 0.0021; *** P < 0.0002; **** P < 0.0001. C Immunoblot of cytosolic or mitochondrial fractions of different cell lines. D Purified mitochondria were digested with increasing concentrations of digitonin, and retained proteins were analyzed by western blot. E Immunogold staining coupled with electron microscopy. Representative stained particles for DHX36 or COX IV in the cytoplasm (C) or mitochondria (M) are indicated with arrows. Scale bars are indicated on images. F LENT levels quantified by RT-qPCR in cytosolic or mitochondrial fractions of different cell lines.

    Journal: Cell Death & Disease

    Article Title: Interaction of lncRNA LENT with DHX36 regulates translation and suppresses autophagy in melanoma

    doi: 10.1038/s41419-025-08296-3

    Figure Lengend Snippet: A Immunofluorescence by confocal microscopy showing DHX36 localization in different cell lines. HSP60 is used as a mitochondria marker and DAPI to stain the nucleus. Scale bars = 10 µM. B DHX36 signal was quantified in the whole cell and divided by the signal in mitochondria or in the nucleus. Each measured cell is represented by one point, and groups are compared by one-way ANOVA (Dunnett test). * P < 0.033; ** P < 0.0021; *** P < 0.0002; **** P < 0.0001. C Immunoblot of cytosolic or mitochondrial fractions of different cell lines. D Purified mitochondria were digested with increasing concentrations of digitonin, and retained proteins were analyzed by western blot. E Immunogold staining coupled with electron microscopy. Representative stained particles for DHX36 or COX IV in the cytoplasm (C) or mitochondria (M) are indicated with arrows. Scale bars are indicated on images. F LENT levels quantified by RT-qPCR in cytosolic or mitochondrial fractions of different cell lines.

    Article Snippet: Then, purified mitochondria were digested on ice for 15 min with increasing concentrations of Digitonin (Invitrogen) in mitochondria isolation buffer (210 mM Mannitol, 70 mM Sucrose, 1 mM EDTA, 10 mM HEPES and protease inhibitors cocktail).

    Techniques: Immunofluorescence, Confocal Microscopy, Marker, Staining, Western Blot, Purification, Electron Microscopy, Quantitative RT-PCR

    A Transmission electron microscopy of 501Mel cells 48 h following transfection of control or LENT-targeting ASO. Autophagosomes and degraded melanosomes were observed in LENT-silenced cells. Scale bars are indicated on the images. B Quantification of cytosolic LC3B (LC3-I) and lipid-associated LC3B (LC3 II) by western blot in 501Mel or MM117 cells. Vinculin is used as a loading control. C LC3B immunoblot in extracts of IGR37 CDX tumors. D Confocal microscopy of unfixed 501Mel cells stained with lysotracker and mitotracker in control or LENT-silenced conditions. The numbers of co-localizing lysosomes and mitochondria was determined and compared between the two conditions by Welch’s test. Scale bars = 10 µM. E Oxygen consumption rate was determined upon LENT or DHX36 silencing. Reserve capacity was obtained by subtracting the maximal capacity with the basal capacity. Comparisons were done by one-way ANOVA (Dunnett test). * P < 0.033; ** P < 0.0021; *** P < 0.0002; **** P < 0.0001.

    Journal: Cell Death & Disease

    Article Title: Interaction of lncRNA LENT with DHX36 regulates translation and suppresses autophagy in melanoma

    doi: 10.1038/s41419-025-08296-3

    Figure Lengend Snippet: A Transmission electron microscopy of 501Mel cells 48 h following transfection of control or LENT-targeting ASO. Autophagosomes and degraded melanosomes were observed in LENT-silenced cells. Scale bars are indicated on the images. B Quantification of cytosolic LC3B (LC3-I) and lipid-associated LC3B (LC3 II) by western blot in 501Mel or MM117 cells. Vinculin is used as a loading control. C LC3B immunoblot in extracts of IGR37 CDX tumors. D Confocal microscopy of unfixed 501Mel cells stained with lysotracker and mitotracker in control or LENT-silenced conditions. The numbers of co-localizing lysosomes and mitochondria was determined and compared between the two conditions by Welch’s test. Scale bars = 10 µM. E Oxygen consumption rate was determined upon LENT or DHX36 silencing. Reserve capacity was obtained by subtracting the maximal capacity with the basal capacity. Comparisons were done by one-way ANOVA (Dunnett test). * P < 0.033; ** P < 0.0021; *** P < 0.0002; **** P < 0.0001.

    Article Snippet: Then, purified mitochondria were digested on ice for 15 min with increasing concentrations of Digitonin (Invitrogen) in mitochondria isolation buffer (210 mM Mannitol, 70 mM Sucrose, 1 mM EDTA, 10 mM HEPES and protease inhibitors cocktail).

    Techniques: Transmission Assay, Electron Microscopy, Transfection, Control, Western Blot, Confocal Microscopy, Staining